What is Q Sepharose?

What is Q Sepharose?

Q Sepharose® Fast Flow is a strong anion exchanger based on the well established Sepharose® Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Is Q Sepharose a cation exchanger?

Q Sepharose Fast Flow is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications. Well-proven strong anion exchanger developed for industrial downstream processes.

How does Q Sepharose work?

The charged group of Q-Sepharose is a quarternary amine which carries a non- titratable positive charge. This matrix can be used at alkaline pH values at which the positive charge of the DEAE group would have been titrated. The charged group of S-Sepharose is the sulphonyl group (-SO3¯).

What is Fast Flow chromatography?

Sepharose 6 Fast Flow is a size exclusion chromatography resin developed for industrial processing at high flow rates and moderate pressures. Industrial-scale separation of large molecules and virus particles. High flow velocities at moderate pressures deliver good resolution with speed.

How do you clean Q Sepharose?

you can easily clean Q sepharose resin with 4-5 culun volume of 2M NaCl then followed by 8M Urea or 7M guanidium hydrochloride and excess for distilled water and try to store your resin with at least 20% ethanol for long term storage. You can never fully clean Q sepharose.

What is Q column?

RESOURCE Q columns are prepacked with SOURCE 15Q, a strong anion exchanger for high resolution polishing purification of proteins at high flow rates. Quaternary ammonium (Q) strong anion exchanger for high resolution ion exchange chromatography.

How do you pack Q Sepharose column?

When packing Sepharose 4 Fast Flow media in INdEX columns to bed heights of 15 and 30 cm, the optimal hydraulic packing pressure is between 0.7 bar and 0.8 bar. The final mechanical compression at the end of the packing procedure should be about 5 mm.

How do you clean a Mono Q column?

Clean the column after filter change according to regular cleaning. over the column 40 ml elution buffer at a flow rate of 2 ml/min through the column. Return to normal flow direction and run for 10 minutes at 4 ml/min. If high back- pressure persists, clean the column.

How do I clean up SP column?

Inject 1 column volume of pepsin (1 mg/mL in 0.5 M NaCl, 0.1 M acetic acid). Leave overnight at room temperature or for one hour at 37 °C. Rinse with at least 2 column volumes of distilled water (Table 21 for recommended flow) until the UV-baseline and the eluent pH are stable.

How do you clean a Q Sepharose column?

What is Monoq?

General description. Mono Q® is a strong anion exchanger prepacked with MonoBeads in a Tricorncolumn. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required.